What efficacy and safety literature is there for use of tirofiban in non-cardioembolic stroke?

Comment by InpharmD Researcher

The latest AHA/ASA guidelines do not recommend intravenous tirofiban for non-cardioembolic stroke; however, limited emerging evidence suggests it may improve short-term neurological function and 90-day outcomes when administered intravenously or intra-arterially, alone or with low-dose rt-PA, without a meaningful increase in symptomatic intracranial hemorrhage or mortality. While promising, these findings are constrained by small sample sizes, heterogeneous patient populations, and variable dosing, leaving overall conclusions uncertain.

Background

According to the 2026 American Heart Association/American Stroke Association (AHA/ASA) Guideline for the Early Management of Patients With Acute Ischemic Stroke, the efficacy of intravenous (IV) tirofiban to improve clinical outcomes in patients with acute ischemic stroke (AIS) is not well established. Although early indications suggest that IV tirofiban may enhance recanalization rates when used with intravenous thrombolysis (IVT), definitive data on long-term safety and functional benefits remain limited. Additional research is needed to determine optimal patient selection, such as those with large-vessel occlusion (LVO) or who are undergoing bridging therapies, to establish dosing regimens, and to clarify the interplay of tirofiban’s platelet inhibition with thrombolytics to mitigate hemorrhagic risk. Ongoing large multicenter trials aim to validate these preliminary safety findings, define net clinical benefit, and refine treatment protocols. In contrast, for patients with noncardioembolic AIS or transient ischemic attack (TIA), antiplatelet therapy is indicated in preference to oral anticoagulation to reduce the risk of recurrent ischemic stroke and other cardiovascular events while minimizing the risk of bleeding. The selection of an antiplatelet agent for early secondary stroke prevention should be individualized based on patient risk factor profiles, cost, tolerance, relative known efficacy of the agents, and other clinical considerations. The use of tirofiban in this context is not detailed or recommended for noncardioembolic stroke. [1]

Given the uncertainty in current guidelines about the efficacy of IV tirofiban for AIS, investigators have assessed its use in combination with reperfusion therapy versus reperfusion therapy alone. A 2025 meta-analysis evaluated seven randomized controlled trials comprising 1,607 patients, of whom 815 received tirofiban with reperfusion therapy and 792 received reperfusion therapy alone. The addition of tirofiban was associated with a higher rate of favorable functional outcomes (risk ratio [RR] 1.25, 95% confidence interval [CI] 1.11 to 1.40; p<0.001) and less functional disability (RR 0.72, 95% CI 0.53 to 0.98; p<0.05). Tirofiban also significantly improved National Institutes of Health Stroke Scale (NIHSS) scores at seven days (mean difference [MD] -2.27, 95% CI -4.32 to -0.22; p= 0.03). Rates of successful revascularization were similar between the tirofiban and control groups (RR 1.18, 95% CI 0.97 to 1.45; p= 0.09). Tirofiban did not significantly increase the risk of symptomatic intracranial hemorrhage (sICH; RR 1.47, 95% CI 0.98 to 2.19; p= 0.06), but there was a higher risk of any intracranial hemorrhage (ICH), particularly in patients undergoing endovascular thrombectomy (EVT; RR 1.25, 95% CI 1.03 to 1.51; p= 0.02). Mortality rates were similar between groups (RR 1.05, 95% CI 0.80 to 1.38; p= 0.72). Overall, the addition of tirofiban to reperfusion therapy improved functional outcomes and NIHSS scores without significantly increasing sICH or mortality, although the risk of any ICH was higher in EVT patients. Importantly, the analysis did not report stroke etiology or stratify results by subtype, so the patient population was likely mixed with respect to cardioembolic and non-cardioembolic strokes; the findings may lack generalizability to non-cardioembolic stroke patients specifically. [2]

Another meta-analysis evaluated the safety and efficacy of tirofiban in patients with AIS. The analysis included 17 studies comprising 2,914 patients and compared outcomes between patients treated with tirofiban and those who did not receive tirofiban. Pooled results showed that tirofiban did not increase the risk of symptomatic intracranial hemorrhage (sICH; odds ratio [OR] 0.95, 95% CI 0.71 to 1.28; p= 0.75) or mortality (OR 0.80; 95% CI 0.64 to 1.02; p= 0.07). However, fatal ICH occurred more frequently in the tirofiban group (OR 2.84; 95% CI 1.38 to 5.85; p= 0.005). Subgroup analysis suggested that intra-arterial administration was associated with an increased risk of fatal ICH (OR 2.90; 95% CI 1.12 to 7.55; p= 0.03), whereas intravenous administration was not (OR 2.75; 95% CI 0.92 to 8.20; p= 0.07). Tirofiban was not associated with a significant improvement in functional outcomes, defined as modified Rankin Scale (mRS) 0 to 2 at three months (OR 1.29; 95% CI 0.97 to 1.71; p= 0.08). Unfortunately, subgroup analyses were performed based on route of administration rather than stroke etiology, limiting the ability to determine whether these findings apply specifically to noncardioembolic stroke. [3]

A 2025 prospective, nonrandomized comparative study evaluated the effectiveness and safety of intra-arterial tirofiban in patients with acute ischemic stroke without large- or medium-vessel occlusion. Sixty patients presenting within 24 hours of symptom onset were allocated by patient preference to receive either intra-arterial tirofiban via catheterization followed by dual antiplatelet therapy or intravenous tirofiban with the same antiplatelet regimen. Both groups experienced significant reductions in National Institutes of Health Stroke Scale scores after treatment; however, the intra-arterial group demonstrated significantly lower scores at 24 hours, 72 hours, and 14 days, as well as improved functional outcomes at 90 days, including lower modified Rankin Scale scores and higher Barthel Index scores. The incidence of adverse events did not differ significantly between groups. Interpretation of these findings is limited because the study used a nonrandomized patient-preference allocation design and is currently available only in abstract form, limiting full evaluation of the methodology and results. [4]

The Clinical Trials database lists the ongoing CHANCE-4 Trial (Tirofiban for Patients With Intracranial Artery Stenosis and High-risk Acute Non-disabling Cerebrovascular Events), a multicenter, double-blind, double-dummy, randomized clinical trial that will evaluate the efficacy and safety of tirofiban compared with placebo for the prevention of recurrent stroke in patients with intracranial artery stenosis and high-risk acute non-disabling cerebrovascular events. Participants will be randomized 1:1 to receive tirofiban or placebo within 24 hours of symptom onset. The tirofiban group will receive an initial infusion of 0.4 mcg/kg/min for 30 minutes (maximum 1 mg), followed by a continuous infusion of 0.1 mcg/kg/min for 48 hours. The primary efficacy outcome will be any new ischemic stroke at 3 months, and the primary safety outcome will be type 3 or 5 bleeding according to the Bleeding Academic Research Consortium (BARC) criteria at 3 months. The trial is currently recruiting with an estimated trial completion date of December 2026; no results have been reported. [5]

References: [1] Prabhakaran S, Gonzalez NR, Zachrison KS, et al. 2026 guideline for the early management of patients with acute ischemic stroke: a guideline from the american heart association/american stroke association. Stroke. Published online January 26, 2026:STR.0000000000000513. doi:10.1161/STR.0000000000000513
[2] de Almeida Monteiro G, Leite M, Gonçalves OR, et al. Efficacy and safety of intravenous tirofiban combined with reperfusion therapy versus reperfusion therapy alone in acute ischemic stroke: a meta-analysis of randomized controlled trials. J Thromb Thrombolysis. 2025;58(4):526-537. doi:10.1007/s11239-025-03094-2
[3] Gong J, Shang J, Yu H, et al. Tirofiban for acute ischemic stroke: systematic review and meta-analysis. Eur J Clin Pharmacol. 2020;76(4):475-481. doi:10.1007/s00228-019-02817-8
[4] Qiu L, Xiong M, Li X, Xu L, Li Z, Ouyang P. Efficacy and safety of intra-arterial tirofiban in acute ischemic stroke without large-vessel occlusion. Neuro Endocrinol Lett. Published online December 29, 2025.
[5] Clinicaltrials.gov. Tirofiban for Patients With intraCranial Artery Stenosis and High-risk Acute Non-disabling Cerebrovascular Events (CHANCE-4). Updated July 12, 2024. Accessed March 5, 2026. https://clinicaltrials.gov/study/NCT06319846
Literature Review

A search of the published medical literature revealed 5 studies investigating the researchable question:

What efficacy and safety literature is there for use of tirofiban in non-cardioembolic stroke?

Level of evidence

C - Multiple studies with limitations or conflicting results  Read more→



Please see Tables 1-5 for your response.


Effects of Tirofiban on Neurological Deterioration in Patients With Acute Ischemic Stroke: A Randomized Clinical Trial
Design

Multicenter, open-label, randomized clinical trial in China

N= 425

Objective To determine whether intravenous (IV) tirofiban administered within 24 hours of stroke onset prevents early neurological deterioration (ND) in patients with acute noncardioembolic stroke compared with oral aspirin
Study Groups

IV tirofiban group (n= 213)

Oral aspirin group (n= 212)

Inclusion Criteria Patients aged 18 to 80 years with acute noncardioembolic stroke within 24 hours of onset and a National Institutes of Health Stroke Scale (NIHSS) score of 4 to 20
Exclusion Criteria

Patients with severe stroke (NIHSS score >20), minor stroke (NIHSS score <4), cardioembolic stroke, those who underwent IV thrombolysis or endovascular thrombectomy, and other specific medical conditions (e.g., malignant tumors, liver cirrhosis, kidney failure, and congestive heart failure)

Methods

Participants were randomly assigned to receive either IV tirofiban (0.4 μg/kg/min for 30 minutes, followed by 0.1 μg/kg/min for 71.5 hours) or oral aspirin for 72 hours. All patients then received oral aspirin. Clinical assessments were performed at baseline, 24, 48, and 72 hours, and on day 7 or at discharge.

Duration

Between September 12, 2020, and March 31, 2023

Treatment: 72 hours,

Follow-up: Up until 90 days

Outcome Measures

Primary: Early ND (increase in NIHSS score ≥4 points) within 72 hours

Secondary: Early ND (NIHSS score increase ≥2 points), early improvement (NIHSS score decrease ≥4 points), level of disability at 90 days (Modified Rankin Score [mRS]), excellent functional outcome (mRS 0-1), functional independence (mRS 0-2), and change in NIHSS score from baseline to 24 hours, 72 hours, and 7 days

Baseline Characteristics   Tirofiban group (n= 213) Aspirin group (n= 212)
Age, median (IQR), years 64 (56-70) 64 (56-71)
Male 154 (72.3%) 147 (69.3%)

Medical history

Hypertension

Diabetes

Cerebral infarction

 

132 (62.0%)

68 (31.9%)

57 (26.7%)

 

134 (63.2%)

67 (31.6%)

59 (27.8%)

Baseline NIHSS score, median (IQR) 5 (4-7) 5 (4-8)

Location of cerebral infarction

Anterior circulation

Posterior circulation

 

126 (59.2%)

47 (22.1%)

 

112 (52.8%)

53 (25%)

BP at randomization, median (IQR), mmHg

Systolic

Diastolic

 

155 (140-168)

88 (79-97)

 

153 (138.5-168)

88 (78.5-99)

Glucose at admission, median (IQR), mmol/L

6.4 (5.4-9.2)

6.4 (5.4-9.0)

Stroke cause

Large-artery atherosclerosis

Cardioembolism

Small-vessel occlusion

Other determined cause

Undetermined cause

 

49 (23.0%)

4 (1.9%)

72 (33.8%)

0

88 (41.3%)

 

65 (30.7%)

5 (2.4%)

52 (24.5%)

3 (1.4%)

87 (41%)

Time from symptom onset to randomization, median (IQR), hours

12.5 (7.8-19.2)  10.5 (6.6-21)

Degree of culprit vessel stenosis

No stenosis

Mild (<50%)

Moderate stenosis (50%-69%)

Severe stenosis (70%-99%)

Occlusion (100%)

 

93/169 (55%)

27/169 (16%)

11/169 (6.5%)

22/169 (13%)

15/169 (8.9%)

 

75/166 (45.2%)

27/166 (16.3%)

19/166 (11.4%)

19/166 (11.4%)

26/166 (15.7%)

Results Endpoint Tirofiban group (n= 213) Aspirin group (n= 212) Measure of effect Adjusted (95% CI) Adjusted p-Value
Primary efficacy endpoint
Early ND within 72 h after randomization 9/213 (4.2%) 28/212 (13.2%) Relative risk (RR) 0.32 (0.16 to 0.65) 0.002
Secondary efficacy endpoints
Early ND (NIHSS score increase ≥2 within 72 h after randomization) 25/213 (11.7) 50/212 (23.6) RR 0.49 (0.32 to 0.75) 0.001
mRS score at 90 d, median (IQR) 1 (0 to 1.25) 1 (0 to 2)

Crude odds ratio (cOR)

1.28 (0.90 to 1.83) 0.17

NIHSS score change from baseline

At 24 h after randomization

At 72 h 

At 7 d or at early discharge

 

−0.33 (1.88)

−0.97 (2.95)

−2.03 (3.07)

 

−0.01 (3.26)

−0.58 (3.77)

−1.74 (3.01)

 

Mean difference (MD)

MD

MD

 

-0.32 (-0.81 to 0.18)

-0.39 (-1.01 to 0.24)

-0.29 (-0.90 to 0.36)

 

0.21

0.22

0.40

Early improvement (NIHSS score decrease ≥4)

28/213 (13.1%)

28/213 (13.1%)

RR

1.13 (0.70 to 1.18)

0.62
Adverse Events No patients in either treatment group experienced symptomatic intracerebral hemorrhage. Three patients (1.3%) in the tirofiban group and three (1.5%) in the aspirin group died by 90 days follow-up. Serious adverse events occurred in 5 patients (2.2%) receiving tirofiban and 4 (2.0%) receiving aspirin.
Study Author Conclusions In patients with noncardioembolic stroke who were seen within 24 hours of symptom onset, tirofiban decreased the risk of early neurological deterioration but did not increase the risk of symptomatic intracerebral hemorrhage or systematic bleeding.
InpharmD Reseracher Critique The study's strengths include its randomized design and multicenter approach, which enhance the generalizability of the findings. However, limitations include the exclusion of patients with severe or minor strokes and those with cardioembolic stroke, which may limit the applicability of the results to all stroke patients. Additionally, the lack of mandatory follow-up imaging may have led to underreporting of asymptomatic intracerebral hemorrhage.
References:
[1] [1] Zhao W, Li S, Li C, et al. Effects of Tirofiban on Neurological Deterioration in Patients With Acute Ischemic Stroke: A Randomized Clinical Trial [published correction appears in JAMA Neurol. 2024 Aug 1;81(8):889. doi:10.1001/jamaneurol.2024.2195]. JAMA Neurol. 2024;81(6):594-602. doi:10.1001/jamaneurol.2024.0868

Tirofiban for Stroke without Large or Medium-Sized Vessel Occlusion

Design

Multicenter, double-blind, double-dummy, randomized clinical trial in China

N= 1177 

Objective

To assess the efficacy and safety of intravenous (IV) tirofiban compared with oral aspirin in patients with acute ischemic stroke without large or medium-sized vessel occlusion

Study Groups

Tirofiban group (n= 606)

Aspirin group (n= 571)

Inclusion Criteria

Adults 18 years or older with acute ischemic stroke, NIHSS score of 5 or more, at least one moderately to severely weak limb, and no large or medium-sized vessel occlusion

Eligible patients had any of four clinical presentations: ineligible for thrombolysis or thrombectomy and within 24 hours after the patient was last known to be well; progression of stroke symptoms 24 to 96 hours after onset; early neurologic deterioration after thrombolysis; or thrombolysis with no improvement at 4 to 24 hours.

Exclusion Criteria

Intracranial hemorrhage, definite cardiac embolism source, large or medium-sized vessel occlusion, NIHSS score less than 5, and other specific medical conditions

Methods

Patients received IV tirofiban (0.4 μg/kg/min for 30 min, then 0.1 μg/kg/min for up to 48 hours) plus oral placebo, or oral aspirin (100 mg/day) plus IV placebo for 2 days, followed by oral aspirin until day 90 for all patients.

Duration

From October 20, 2020, through June 30, 2022

90 days follow-up

Outcome Measures

Primary: Excellent outcome (modified Rankin scale score of 0 or 1) at 90 days
Secondary: Functional independence at 90 days, quality-of-life score, global outcome analysis, level of disability

Baseline Characteristics    Tirofiban Group (n= 606) Aspirin Group (n= 571)
Median age (IQR), years 68.0 (58.0–75.0) 68.0 (59.0–76.0)
Male  379 (62.5) 373 (65.3)
NIHSS score, median (IQR) 9.0 (7.0–10.0) 9.0 (7.0–10.0)
Median Alberta Stroke Program Early Computed Tomography Score (ASPECTS) (IQR) 9.0 (9.0–10.0) 9.0 (9.0–10.0)
Median systolic blood pressure at hospital arrival (IQR), mm Hg 155 (142–166) 156 (144–167)
Median glucose level at hospital arrival (IQR), mmol/L 6.6 (5.6–8.5) 6.4 (5.4–8.7)

Presentation type

Ineligible for reperfusion treatment and within 24 hr after stroke onset

Ineligible for reperfusion treatment and progression 24–96 hr after stroke onset

IV thrombolysis (IVT) followed by early neurologic deterioration

IVT followed by no neurologic improvement

 

332 (54.8%)

199 (32.8%)

45 (7.4%)

30 (5.0%) 

 

318 (55.7%)

180 (31.5%)

45 (7.9%)

28 (4.9%)

Localization of presenting deficit 

Anterior circulation

Posterior circulation

Anterior circulation plus posterior circulation

Unknown

 

489 (80.7%)

92 (15.2%)

5 (0.8%)

20 (3.3%)

 

456 (79.9%)

94 (16.5%)

7 (1.2%)

14 (2.5%)

Median time from stroke onset or progression of stroke symptoms to randomization (IQR), hours 10.9 (7.2–16.1) 11.2 (7.4–16.8)
Median time from stroke onset or progression of stroke symptoms to initial treatment (IQR), hours 11.3 (7.5–16.5) 11.5 (7.8–17.1)
Results   Tirofiban Group (n =606) Aspirin Group (n =571) Effect Measure Effect Value (95% CI) p-Value
Primary efficacy endpoint
Score of 0 or 1 on the modified Rankin scale at 90 days 176/604 (29.1%) 126/567 (22.2%) Risk ratio (RR) 1.26 (1.04–1.53) 0.02
Secondary efficacy endpoints
Global outcome at 90 days - - Common odds ratio (OR) 1.38 (1.07–1.78) 0.01
Median score on the modified Rankin scale at 90 days (IQR) 2 (1–3) 2 (2–3) Common OR 1.23 (1.00–1.51) 0.06 
Score of 0, 1, or 2 on the modified Rankin scale at 90 days 375/604 (62.1%) 320/567 (56.4%) RR 1.07 (0.98–1.16)
Median EQ-5D-5L score at 90 days (IQR)* 0.83 (0.64–0.93) 0.78 (0.56–0.84) Win ratio  1.40 (1.23–1.62)
Score of 0 or 1 on the modified Rankin scale at 30 days 139/605 (23.0%) 96/568 (16.9%) RR 1.29 (1.03–1.62)
Score of 0, 1, or 2 on the modified Rankin scale at 30 days 307/605 (50.7%) 263/568 (46.3%) RR 1.06 (0.95–1.18) -
Primary safety endpoints
Death 23/604 (3.8%) 15/567 (2.6%) Adjusted RR 1.62 (95% CI, 0.88 to 2.95) 0.12
Symptomatic intracranial hemorrhage, as defined in HBC criteria 6 (1.0%) 0 - - 0.03
Secondary safety endpoints
Intracranial hemorrhage on any imaging 6 (1.0%)  0 - - 0.03
Serious adverse event (AE) 97 (16.0) 74 (13.0) - - 0.14 
Any AE 380 (62.7) 349 (61.1) 0.58 
Severe bleeding event 9 (1.5) 1 (0.2)
Adverse Events

See Results

Study Author Conclusions

Intravenous tirofiban was associated with a greater likelihood of an excellent outcome at 90 days than low-dose aspirin in patients with acute ischemic stroke without large or medium-sized vessel occlusion. Incidences of intracranial hemorrhages were low but slightly higher with tirofiban.

InpharmD Researcher Critique

The study's strengths include a large sample size and rigorous design. Limitations include the heterogeneity of patient presentations and the limited generalizability to populations with cardioembolic strokes and other racial ethnicities. The study did not require follow-up imaging in the absence of neurologic worsening, which may limit the detection of asymptomatic hemorrhagic transformation. 

Half of the presentation types were ineligible for reperfusion treatment, yet the results were not further stratified based on the clinical presentations. 

References:
[1] [1] Zi W, Song J, Kong W, et al. Tirofiban for Stroke without Large or Medium-Sized Vessel Occlusion. N Engl J Med. 2023;388(22):2025-2036. doi:10.1056/NEJMoa2214299

 

Efficacy and safety of tirofiban combined with endovascular therapy for basilar artery occlusion stroke due to large artery atherosclerosis
Design

Non-randomized, multicenter study using data from the Endovascular Treatment for Acute BASILAR Artery Occlusion (BASILAR) registry

N= 417

Objective

To evaluate the efficacy and safety of adjuvant tirofiban in patients with acute basilar artery occlusion due to large-artery atherosclerotic (LAA) receiving endovascular therapy (EVT)

Study Groups

Tirofiban group (n= 275)

Non-tirofiban group (n= 142)

Inclusion Criteria

Patients with acute basilar artery occlusion due to LAA within 24h of symptom onset who underwent EVT

Exclusion Criteria

Exclusion criteria were not explicitly stated

Methods

Patients were divided into tirofiban and non-tirofiban groups based on the use of tirofiban. Intravenous tirofiban was administered in cases of recurrent stenosis, instant re-occlusion, or suspected LAA. EVT techniques included stent-retrievers, aspiration, intra-arterial thrombolysis, balloon angioplasty, stenting angioplasty, and combination therapies.

Duration

January 2014 to May 2019

Outcome Measures

Primary: Ordinal modified Rankin scale score at 90 days

Secondary: Mortality within 90 days, symptomatic intracranial hemorrhage (sICH) within 48 h

Baseline Characteristics   Non-tirofiban (n= 142) Tirofiban (n= 275)
Age, years, median (IQR) 63 (54-69) 63 (56-70)
Male 122 (85.9%) 222 (80.7%)
History of smoking 73 (51.4%) 113 (41.1%)
General anesthesia 37 (26.6%) 133 (48.7%)
Balloon angioplasty and/or stenting 70 (49.3%) 194 (70.8%)
IQR= interquartile range 
Results   Non-tirofiban (n= 142) Tirofiban (n= 275) p-Value
mRS score at 90 days, median (IQR) 6 (4-6) 5 (2-6) 0.002
Successful recanalization at final angiogram 98 (69.0%) 240 (87.3%) <0.001
Any ICH within 48 h 22 (15.8%) 19 (7.1%) 0.005
sICH within 48 h 17 (12.2%) 14 (5.2%) 0.011
Mortality within 90 days 83 (58.5%) 112 (40.7%) 0.001
Adverse Events The rate of symptomatic intracranial hemorrhage (sICH) was lower in the tirofiban group (5.2%) compared to the non-tirofiban group (12.2%)
Study Author Conclusions Tirofiban plus EVT might improve functional outcomes with a good safety profile for patients with acute basilar artery occlusion due to LAA. The results need to be confirmed in a randomized trial. 
Critique The study's non-randomized design may introduce selection bias, and the lack of uniform tirofiban dosing protocols across centers could affect the results. Additionally, the study population was limited to a Chinese cohort, which may limit the generalizability of the findings to other ethnic groups.
References:
[1] [1] Zhu X, Guo Z, Tian L, et al. Efficacy and safety of tirofiban combined with endovascular therapy for basilar artery occlusion stroke due to large artery atherosclerosis. J Stroke Cerebrovasc Dis. 2024;33(2):107526. doi:10.1016/j.jstrokecerebrovasdis.2023.107526

 

Safety and efficacy of low-dose rt-PA with tirofiban to treat acute non-cardiogenic stroke: a single-center randomized controlled study
Design

Single-center, randomized, open label, experimental study

N= 60

Objective To investigate the effectiveness and safety of infusing tirofiban after intravenous thrombolysis (IVT) with low-dose rt-PA in patients with acute non-cardiogenic ischemic stroke
Study Groups

rt-PA + T group (n= 30)

rt-PA group (n= 30)

Inclusion Criteria Age ≥18 years; hospitalized within 4.5 h after onset of clinical symptoms; clinical diagnosis consistent with acute ischemic stroke; no atrial fibrillation or severe heart disease; NIHSS score <15; craniocerebral CT performed to eliminate intracranial hemorrhage; informed consent obtained
Exclusion Criteria Contraindications to thrombolysis or any of the inclusion criteria not met
Methods Patients were randomly divided into two groups: rt-PA + T group received low-dose rt-PA (0.6 mg/kg) and tirofiban, while rt-PA group received standard dose rt-PA (0.9 mg/kg). Tirofiban was administered at 0.4 µg/kg/min for 30 min, then 0.1 µg/kg/min for 24 h. Safety and efficacy were assessed using NIHSS and mRS scores
Duration January 2018 to December 2019
Outcome Measures

Primary: Safety (sICH, any ICH, severe systemic bleeding, mortality)

Secondary: Curative efficacy (7d-NIHSS score, functional outcomes at 90 days)

Baseline Characteristics   rt-PA + T group (n= 30) rt-PA group (n= 30) P-value
Age 65.33 ± 8.18 66.53 ± 10.27 0.619
Gender(male) (n, %) 20 (66.7%) 18 (60.0%) 0.592
Smoking (n, %) 10 (33.3%) 11 (36.7%) 0.787
Drinking (n, %) 10 (33.3%) 9 (30.0%) 0.781
Hypertension (n, %) 20 (66.7%) 19 (63.3%) 0.787
Diabetes Mellitus (n, %) 7 (23.3%) 5 (16.7%) 0.519
Total platelets (x10^9/L) 253.83 ± 11.94 249.30 ± 11.58 0.141
Total cholesterol (mmol/L) 4.71 ± 0.99 4.75 ± 1.23 0.903
Triglyceride (mmol/L) 1.29 ± 0.57 1.06 ± 0.51 0.102
LDL (mmol/L) 2.88 ± 0.79 2.88 ± 1.02 0.997
HCY (µmol/L) 11.78 ± 3.13 12.11 ± 2.70 0.670
Admission NIHSS 7.63 ± 3.54 7.37 ± 3.88 0.782
Results   rt-PA + T group (n= 30) rt-PA group (n= 30) P-value
sICH (n, %) 0 0 -
Any ICH (n, %) 3 (10) 1 (3.3) 0.306
Systemic bleeding (n, %) 0 0 -
Mortality (n, %) 0 0 -
END (n, %) 0 2 (6.6) 0.246
No END (n, %) 30 28 (93.4) 0.246
7d-NIHSS score 2.33 ± 1.85 4.80 ± 4.02 0.004
90-day favorable functional outcome (mRS 0-2) 25 (83.3%) 18 (60.0%) 0.045
Adverse Events No increase in symptomatic intracranial hemorrhage (sICH), any ICH, severe systemic bleeding, or mortality between groups.
Study Author Conclusions Low-dose rt-PA combined with tirofiban in acute non-cardiogenic ischemic stroke did not increase the risk of ICH and mortality, and was associated with neurological improvement.
Critique The study's strengths include its randomized controlled design and clear demonstration of safety and efficacy of low-dose rt-PA with tirofiban. However, the small sample size and lack of neuroimaging to confirm arterial occlusion type are limitations. Additionally, the exclusion of cardiogenic stroke based only on ECG and medical history may introduce bias. Further large-scale studies are needed to validate these findings.
References:
[1] [1] Liang Z, Zhang J, Huang S, et al. Safety and efficacy of low-dose rt-PA with tirofiban to treat acute non-cardiogenic stroke: a single-center randomized controlled study. BMC Neurol. 2022;22(1):280. Published 2022 Jul 27. doi:10.1186/s12883-022-02808-w

 

Thrombolysis with Adjunctive Tirofiban in Acute Non-cardioembolic Posterior Circulation Ischemic Stroke: A Propensity Score-Matched Analysis
Design

Retrospective, single-center cohort study with propensity score matching

N= 256

Objective To determine whether adjunctive tirofiban, combined with standard rt-PA thrombolysis, improves 90-day functional outcomes without increasing major bleeding risks in patients with acute non-cardioembolic posterior circulation ischemic stroke treated within 24 hours of onset
Study Groups

rt-PA + T group (n= 128)

rt-PA group (n= 128)

Inclusion Criteria Age ≥18 years; imaging confirmation of infarction within the vertebrobasilar artery territory; administration of standard-dose intravenous alteplase within 24 hours from symptom onset; admission NIHSS score ≤20; classification of ischemic stroke etiology as non-cardioembolic according to CISS criteria
Exclusion Criteria Receipt of endovascular therapy; contraindications to thrombolysis or tirofiban; use of other investigational treatments; absence of essential data required for analysis
Methods Patients in the rt-PA + T group received standard intravenous alteplase thrombolysis followed by intravenous tirofiban. Tirofiban was administered as an intravenous bolus at 0.4 μg/kg/min for 30 min, followed by continuous infusion at 0.1 μg/kg/min for 24–48 hours. The rt-PA group received standard-dose intravenous rt-PA alone. Propensity score matching was used to create well-balanced cohorts
Duration January 1, 2020, to May 1, 2025
Outcome Measures

Primary: Favorable functional outcome (mRS 0–1) at 90 days

Secondary: Functional independence (mRS 0–2) at 90 days, early neurological deterioration, early neurological improvement

Baseline Characteristics   rt-PA + T group (n= 128) rt-PA group (n= 128)
Age, years 66.77 ± 10.17 65.68 ± 11.38
Male, n (%) 86 (67.19) 90 (70.31)
Hypertension, n (%) 100 (78.12) 99 (77.34)
Diabetes, n (%) 52 (40.62) 53 (41.41)
Coronary artery disease, n (%) 21 (16.41) 19 (14.84)
Previous stroke or TIA, n (%) 23 (17.97) 29 (22.66)
Smoke, n (%) 56 (43.75) 67 (52.34)
Alcohol, n (%) 41 (32.03) 50 (39.06)
SBP, mmHg 162.12 ± 24.97 159.88 ± 23.05
DBP, mmHg 89.41 ± 14.54 89.09 ± 14.64
NIHSS at admission, score 5.00 (2.00, 8.00) 3.00 (2.00, 6.00)
Results   rt-PA + T group (n= 128) rt-PA group (n= 128) OR (95% CI) p-Value
mRS score 0–1 at 90 days, n (%) 81 (63.3) 64 (50.0) 1.72 (1.05–2.84) 0.033
mRS score 0–2 at 90 days, n (%) 94 (73.4) 78 (60.9) 1.77 (1.04–3.01) 0.034
Early neurological deterioration, n (%) 10 (7.8) 9 (7.0) 1.10 (0.43- 2.81) 0.839
Early neurological improvement, n (%) 24 (18.8) 30 (23.4) 0.75 (0.41–1.38) 0.359
Symptomatic intracranial hemorrhage, n (%) 2 (1.6) 0 (0.0) 5.08 (0.24–106.01) 0.498
Any bleeding event, n (%) 8 (6.3) 7 (5.5) 1.46 (0.54–3.98) 0.454
All-cause mortality within 90 days, n (%) 3 (2.3) 2 (1.6) 1.51 (0.25–9.20) 0.654
Adverse Events No significant differences in symptomatic intracranial hemorrhage (1.6% vs. 0.0%, p= 0.498), any bleeding (6.3% vs. 5.5%, p= 0.454), or 90-day mortality (2.3% vs. 1.6%, p= 0.654)
Study Author Conclusions Adjunctive tirofiban following standard intravenous thrombolysis was associated with significantly improved 90-day functional outcomes without a notable increase in major bleeding risk, particularly in patients with lower baseline NIHSS scores.
Critique The study provides valuable real-world evidence supporting the use of adjunctive tirofiban in acute non-cardioembolic posterior circulation ischemic stroke. However, the non-randomized design and reliance on clinician discretion for treatment decisions introduce potential selection bias. The sample size for key exploratory subgroups was limited, reducing the precision of estimates. Additionally, the lack of standardized tirofiban dosing and infusion duration may introduce treatment heterogeneity. Despite these limitations, the study's findings are promising and warrant further investigation through prospective, randomized controlled trials.
References:
[1] [1] Yuan H, Du M, Zhang T, Luan Z, Sun Z, Liang Z. Thrombolysis with Adjunctive Tirofiban in Acute Non-cardioembolic Posterior Circulation Ischemic Stroke: A Propensity Score-Matched Analysis. Neurol Ther. Published online January 19, 2026. doi:10.1007/s40120-026-00885-4